Protein-protein interaction assays: eliminating false positive interactions
نویسندگان
چکیده
منابع مشابه
Proteins interacting with cloning scars: a source of false positive protein-protein interactions
A common approach for exploring the interactome, the network of protein-protein interactions in cells, uses a commercially available ORF library to express affinity tagged bait proteins; these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as putative interacting proteins using mass spectrometry. Control experiments can be used to limit ...
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Protein-protein interactions contribute to a variety of biological processes, including signal transduction, tissue integrity, and force generation (1,2). An inexpensive and reliable method to measure protein-protein interactions would be of considerable utility. Currently, co-immunoprecipitations (Co-IP) are routinely used to detect protein-protein interactions in cells, where one antibody is ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2006
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0206-135